Two months ago I hooked up jsmin from a build script for release configurations. It's been helpful to have all of the reader-friendly stuff stripped out automatically without having to store minified copies in source control.
I wrote a couple of articles a while ago about minifying css/javascript on the fly. They utilise a mod_perl handler which intercepts outgoing css/js and compresses them on the way out.
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http://elsdoerfer.name/docs/django-assets/
http://dojotoolkit.org/docs/shrinksafe
http://hg.errant.me.uk/minify
There is also a port (or rewrite) for Kohana V3 that someone else maintains http://github.com/zazu/minify
https://secure.grepular.com/Compressing_CSS_on_the_Fly
https://secure.grepular.com/Compressing_JavaScript_on_the_Fl...