pyGeno has been recently ported to python 3. It allows you to play with genomes, proteomes, exomes, etc.. locally, in python and with a simple interface. You can use reference genomes or create you own (ex: patient specific) from a reference and sets of mutations.
It's available on github and pypi (pip install pyGeno)
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[ 5.9 ms ] story [ 11.7 ms ] threadIt's available on github and pypi (pip install pyGeno)